Laboratories that prefer to synthesize their own oligonucleotides should consider Nagahama's Dr. Oligo synthesizer. Dr. Oligo juggles the most complex nucleic acid sequence requirements with ease. Dr. Oligo instruments are fully supported by Nagahama's expertise in custom oligonucleotide synthesis.

A brief description and specifications for the Dr. Oligo synthesizer appear below. For more information or to arrange a purchase, please email your contact information and request to tak.nbl.jpn@ksf.biglobe.ne.jp
or telephone [+81] 90-2195-3458.

Dr. Oligo® Synthesizer

The Dr. Oligo synthesizer is a high-throughput synthetic chemistry instrument with primary application to automation of phosphoramidite chemistry for synthesis of DNA and RNA oligomers. This instrument can produce high-quality synthetic oligonucleotides at a rate of 192 x 20-base length per 2.5-3.0 hour period.

Specifications

Chemistry: standard phosphoramidite solid-phase. Format: solid support, usually controlled-pore glass (CPG), contained in 96-well microplates. Capacity: one or two plates; up to 192 oligonucleotides synthesized simultaneously. Scales: 20 nmole to 1 µmole, software controlled, one scale per plate. Amidite reservoirs: 6 included; A,C,G & T plus two for specials or mixed sites. Mixed sites may also be created by program with both spare amidite reservoirs used for specials. Additional reservoirs available as option. Trityl monitoring: Possible using offline 540 nm plate reader (not included). Instrument software supports automatic pause and collection of samples for analysis during selected deblock steps. Control: Dedicated PC running Microsoft Windows 95, 98, NT 4.0, Win 2000 or Win XP. Manual controls to vent and pressurize reservoirs and set gas pressure. Power requirements: Available either 120 VAC or 220 VAC. Included power-interrupt-safety supply operates instrument for 30 minutes in event of power outage. Gas requirements: Inert gas supply, argon recommended. (Nitrogen or helium may also be used.) Liquid gas boil-off system may be used. Gas consumption during operation is 1.2 L per minute. Space requirement: All instrument components cart-mounted. Depth 24 inches, width 68 inches, height 70 inches. Additional 18 inches must be allowed at each end of instrument to allow for movement of sliding plate. Waste: Collected in 5-gallon container with secondary spill container. Bottom spigot allows easy draining into larger waste drum. Protocols: Included for 200 nmole and 50 nmole synthesis scales. May be easily modified or written by operator using ingrated protocol editor. Sequences: Sequence editor included. Sequences are individual text files which may be located on the instrument computer or on network server. Reaction systems: Three different solid-support systems available include fritted plate, rack for ABI-3900-style columns, and rack for CTGen HP CPG frits. The CPG frit option is recommended for best performance.
All components of the Dr. Oligo synthesizer are mounted on one cart, shown above, including chemical reservoirs, waste container, computer, keyboard, monitor, mouse, power supply and control system.
 

The Dr. Oligo synthesizer is able to synthesize on one or two 96-place synthesis plates simultaneously.
 

Reaction System Table
Reagent consumption shown is volume per cycle per oligonucleotide. The synthesis scale is 200 nmoles. Amidite concentraton if 100 mM. Activator is ETT or DCI, and the concentration is 250 mM.
Reaction System: Fritted
Plate		 Rack for 3900-
Style Columns		 Rack with CTGen
HP CPG Frits
Cycle Time, 1 plate: 7.5 min 5.5 min 5.5 min
Cycle Time, 2 plates: 8.75 min 6.5 min 6.5 min
Wash: 1.55 mL 460 µL 600 µL
Deblock: 320 µL 320 µL 320 µL
Activator: 90 µL 45 µL 45 µL
Amidite: 100 µL 30 µL 30 µL
Cap-A: 85 µL 40 µL 40 µL
Cap-B: 85 µL 40 µL 40 µL
Oxidizer: 85 µL 60 µL 60 µL

Dr. Oligo Synthesizer is licensed under U.S. Patents 5,368,823 and 5,541,314; European Patent 0683790; and Japan Patent 6-518236.